Troubleshooting Common Issues with Semi-Automatic Peptide Synthesizer

　　Semi-automatic peptide synthesizer is a commonly used equipment for peptide drug R&D and production, and its operational stability directly affects the synthesis efficiency and product quality. In daily use, the equipment may be abnormal due to operation, environment or component wear and tear, and it is especially important to master the troubleshooting methods of common problems.

　　First, the synthesis efficiency decreases or failure 

If the purity of the synthesized product decreases, the yield does not meet the expectations, you need to prioritize the check of the reagent supply system. If the purity of the synthesized product decreases and the yield is not as expected, check the reagent supply system first. Insufficient resin expansion may lead to incomplete coupling, you need to confirm whether the resin pretreatment step is standardized; reagent pipeline blockage or air bubbles will reduce the contact between the reagent and the resin, you need to check whether the pipeline is smooth, and eliminate air bubbles through the exhaust operation. In addition, abnormal temperature control may affect the coupling reaction rate, need to observe whether the temperature control module is stable.

　　Abnormal pressure fluctuation 

If the pressure alarm or fluctuation occurs during the operation of the semi-automatic peptide synthesizer, it may be related to the sealing of the pipeline or the status of the valve. or valve status. Check whether the pipeline joints are loose, whether the sealing ring is aging, tighten or replace in time; if the valve is stuck or not completely closed, it will lead to pressure imbalance, you need to manually test whether the valve switch is smooth. If the problem persists, need to check whether the pressure sensor failure.

　　Third, program interruption or control failure 

synthetic program suddenly stops or parameter display abnormality, may be the electrical connection or software system problems. Check whether the power supply is stable, whether the control panel keys respond; if the screen reports an error, try to restart the device and reload the program; after long-term use, the control board interface oxidation or software version is too old may also lead to failure, you need to contact the engineer to upgrade the system or clean the interface.

　　Resin leakage or blockage 

Resin leakage is mostly due to the seal failure of the reaction column, you need to reinstall the reaction column and replace the seals; if the filtration step is blocked after synthesis, it may be caused by resin fragments or undissolved resin. If the filtration step is clogged after synthesis, it may be due to resin fragments or undissolved reagent residues, you need to check whether the filter membrane is broken, and ensure that the reagent is completely dissolved before synthesis.

　　V. Daily Maintenance Suggestions 

Regular cleaning of pipelines and reaction chamber, timely replacement of aging seals, calibration of temperature control and pressure sensors can reduce the frequency of failure. Strictly follow the SOP during operation to avoid problems caused by human errors.

　　Troubleshooting of semi-automatic peptide synthesizer should be analyzed step by step from reagent, mechanical and electrical aspects, taking into account the structure and operation logic of the equipment. Timely detection and solution of common problems can not only protect the experimental efficiency, but also prolong the life of the equipment.