Release Time:2024-03-04
Number of views:268
Peptide synthesis is an important research tool in the field of biochemistry and drug discovery, and traditional peptide synthesis methods usually require a lot of manual operation and time. With the development of science and technology, the automated synthesis method based on Peptide Synthesis Instrument is gradually attracting attention.
I. Basic Principle
Peptide Synthesis Instrument is a specialized device for the automated synthesis of peptide compounds, the basic principle of which is to form peptide chains by linking amino acids in a predetermined sequence through chemical reactions. It usually consists of the following components:
1. Amino acid injector: for storing and injecting amino acids.
2. Protectant injector: used to store and inject a protectant to protect the side chains of the amino acids from reaction.
3. deprotectant syringe: for storing and injecting deprotectant to remove the protective agent from the side chain of the amino acid.
4. Linker syringe: for storing and injecting linker to promote the linking reaction between amino acids.
5. Detergent injector: for storing and injecting detergent to clean the reactor and column.
6. Controller: used to control each component to realize automated synthesis.
II.Based on the automated synthesis method mainly includes the following steps:
1. amino acid activation: activation of amino acids in the amino acid injector to form active esters for attachment to peptide chains.
2. Amino acid linkage: the activated amino acid is injected into the reactor to have a linkage reaction with the terminal amino acid of the peptide chain to form a new peptide chain.
3. Protectant Removal: Inject the deprotectant from the deprotectant syringe into the reactor to remove the protectant from the amino acid side chain.
4. Linker removal: inject the cleaning agent in the cleaner syringe into the reactor, clean the reactor and column to remove the residual linker.
5. Washing and drying: The reactor and column are washed and dried several times to ensure the purity of the peptide chain.
6. Peptide chain separation and collection: Separate the synthesized peptide chain from the reactor for further purification and collection.
Advantages of automated synthesis method
1. Improve efficiency: automated synthesis method can greatly reduce the time and labor intensity of manual operation and improve the efficiency of peptide synthesis.
2. Improve the quality: automated synthesis method can accurately control the reaction conditions, reduce errors and improve the quality of peptide synthesis.
3. Flexibility: The automated synthesis method can adjust the length of peptide chain and amino acid sequence according to the need to meet different research needs.
The automated synthesis method based on Peptide Synthesis Instrument has high application value in the field of peptide synthesis. Operators should choose the appropriate operation method according to specific needs and conditions to ensure the efficiency and quality of peptide synthesis.
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