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Explanation of the automated synthesis process and operation steps of the customized peptide synthesizer

Release Time:2024-03-18

Number of views:129

  Customized Peptide Synthesizer is a specialized equipment for synthesizing specific peptides, which can automate the peptide synthesis process according to the user's needs.


  I. Automated Synthesis Process

  It mainly includes the following steps:

  1. Peptide design: according to the user's needs, the desired peptide sequence is designed. This step usually requires professional bioinformatics software to assist the design.


  2. Synthesis scheme development: according to the peptide sequence, develop a corresponding synthesis scheme. The synthesis scheme includes the chemical reaction, reaction conditions, catalyst and other information.


  3. Preparation of raw materials: according to the synthesis scheme, prepare the required raw materials such as amino acids, protective agents and catalysts. The raw materials need to have high purity to ensure the quality of the synthesized products.


  4. Peptide synthesis: Input the designed peptide sequence into the instrument, and the equipment will automatically complete the peptide synthesis process according to the synthesis scheme. This step usually includes multiple cycles, each cycle includes amino acid activation, amino acid coupling, protective agent removal and other steps.


  5. Product purification: After the peptide synthesis is completed, the product needs to be purified to remove unreacted raw materials, catalysts and other impurities. The purification methods usually include column chromatography, centrifugation, dialysis and so on.


  6. Product identification: identify the purified peptide product to confirm its structure and purity. Identification methods usually include mass spectrometry, nuclear magnetic resonance, ultraviolet absorption and so on.


  Analysis of operation steps

  When using customized peptide synthesizer for peptide synthesis, you need to operate according to the following steps:

  1. Peptide design: utilize bioinformatics software to design the required peptide sequence.


  2. Synthesis program development: refer to relevant literature and experimental experience to develop a suitable synthesis program.


  3. Raw material preparation: Purchase the required raw materials such as amino acid, protectant and catalyst, and purify them.


  4. Equipment initialization: connect it to the computer and install the corresponding control software. Set up the experimental parameters, such as reaction volume, reaction time, reaction temperature and so on.


  5. Load raw materials: Load the prepared raw materials into the synthesizer, including amino acids, protective agents, catalysts, etc.


  6. Start synthesis: After confirming the experimental parameters are correct, start the instrument and the equipment will automatically complete the peptide synthesis according to the set synthesis scheme.


  7. Product purification: after the completion of peptide synthesis, the product will be purified to remove impurities.


  8. Product identification: identify the purified peptide product to confirm its structure and purity.


  9. Experimental record: record the experimental process and results for subsequent analysis and reference.


  Customized peptide synthesizer provides a convenient means of peptide synthesis for biological research and drug development. In the process of using, users need to be familiar with the operation steps and synthesis process of the equipment to ensure the success of the experiment and the reliability of the results.

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